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First published online February 21, 2025

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Measurement of adhesive strength between the epidermal and inner tissues of plant stems using a tensile tester

Yuma Shimizu, Kazuyuki Wakabayashi https://orcid.org/0000-0003-2257-434X, […], Kensuke Miyamoto, and Kouichi Soga https://orcid.org/0000-0002-7088-1734 [email protected]+1View all authors and affiliations

Volume 60, Issue 1-2

https://doi.org/10.1177/0006355X241296333

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Abstract
References

Abstract

Background

The plant stem is composed of epidermal and inner tissues that are under tension and compression, respectively. Therefore, the adhesion of both tissues is considered to be involved in the structural integrity of the stem. However, the role of tissue adhesion in stem structure is unclear.

Objective

This study aimed to develop a method for quantitatively measuring the adhesive strength between the epidermal and inner tissues using a tensile tester to determine the possible role of tissue adhesion in stem integrity.

Methods

The epidermal tissue was partially peeled from the segment of pea epicotyls using forceps to create a peeling arm. The peeling arm and the segment region of the partially removed epidermal tissue were fixed to the upper and lower clamps, respectively. By raising the upper clamp at various speeds, the epidermal tissue was peeled from the segment, and the peeling force was recorded.

Results

Adhesive strength was defined as the peeling force normalized by the width of the peeled epidermal tissue. The peeling rate was determined as 100 mm/min. The adhesive strength in the elongation region of the stem was substantially smaller than in the non-elongation region.

Conclusions

A method for quantitatively measuring the adhesive strength between the epidermal and inner tissues was developed. Analysis using this method suggests that adhesive strength may be involved in regulating stem growth.

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References

1. Carpita NC, Gibeaut DM. Structural models of primary cell walls in flowering plants: consistency of molecular structure with the physical properties of the walls during growth. Plant J 1993; 3: 1–30.

2. Cosgrove DJ. Building an extensible cell wall. Plant Physiol 2022; 189: 1246–77.

3. Jarvis MC, Briggs SPH, Knox JP. Intercellular adhesion and cell separation in plants. Plant Cell Environ 2003; 26: 977–89.

4. Heyn ANJ. Der Mechanismus der Zellstreckung. Rec Trav Bot Néerl 1931; 28: 113–244.

5. Tagawa T, Bonner J. Mechanical properties of the Avena coleoptile as related to auxin and to ionic interactions. Plant Physiol 1957; 32: 207–12.

6. Cleland R. A separation of auxin-induced cell wall loosening into its plastic and elastic components. Physiol Plant 1958; 11: 599–609.

7. Olson AC, Bonner J, Morré DJ. Force extension analysis of Avena coleoptile cell walls. Planta 1965; 66: 126–34.

8. Kawamura Y, Hoson T, Kamisaka S,. et al. Formulation of pre-extension in a practical stress-relaxation measurement of the plant cell wall. Biorheol 1995; 32: 611–20.

9. Yamamoto R. Stress relaxation property of the cell wall and auxin-induced cell elongation. J Plant Res 1996; 109: 75–84.

10. Taiz L. Plant cell expansion: Regulation of cell wall mechanical properties. Ann Rev Plant Physiol Plant Mol Biol 1984; 35: 585–657.

11. Cosgrove DJ. Biophysical control of plant cell growth. Annu Rev Plant Physiol 1986; 37: 377–405.

12. Masuda Y. Auxin-induced cell elongation and cell wall changes. Bot Mag Tokyo 1990; 103: 345–70.

13. Hejnowicz Z, Sievers A. Tissue stresses in organs of herbaceous plants III. Elastic properties of the tissues of sunflower hypocotyl and origin of tissue stresses. J Exp Bot 1996; 47: 519–28.

14. Niklas KJ, Paolillo DJ. The role of the epidermis as a stiffening agent in Tulipa (Liliaceae) stems. Am J Bot 1997; 84: 735–44.

15. Kutschera U, Niklas KJ. The epidermal-growth-control theory of stem elongation: An old and a new perspective. J Plant Physiol 2007; 164: 1395–409.

16. Bartlett MD, Case SW, Kinloch AJ,. et al. Peel tests for quantifying adhesion and toughness: A review. Prog Mater Sci 2023; 137: 101086.

17. Goodman AM, Ennos AR, Booth I. A mechanical study of retting in glyphosate treated flax stems (Linum usitatissimum). Ind Crops Prod 2002; 15: 169–77.

18. Kutschera U. The role of the epidermis in the control of elongation growth in stems and coleoptiles. Botanica Acta 1992; 105: 246–52.

19. Van Overbeek J, Went FW. Mechanism and quantitative application of the pea test. Bot Gaz 1937; 99: 22–41.

20. Grillet AM, Nicholas B, Wyatt NB, et al. Polymer gel rheology and adhesion. In: De Vicente J editor. Rheology. InTech; 2012. pp 59–80.

21. Miyagi Z. Consideration of evaluation methods and dependability for properties of adhesion and PSA. J Jpn Soc Colour Materi 2015; 88: 321–5.

22. Réquilé S, Le Duigou A, Bourmaud A,. et al. Peeling experiments for hemp retting characterization targeting biocomposites. Ind Crops Prod 2018; 123: 573–80.

23. Kinloch AJ, Lau CC, Williams JG. The peeling of flexible laminates. Int J Frac 1994; 66: 45–70.

24. Kutschera U, Briggs WR. Growth, in vivo extensibility, and tissue tension in developing pea internodes. Plant Physiol 1988; 86: 306–11.

25. Miyamoto K, Kamisaka S. Effect of gibberellic acid on epicotyl growth and carbohydrate distribution in derooted Pisum sativum cuttings with or without cotyledons. Physiol Plant 1990; 80: 357–64.

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